Homogenizers and Immersion Blenders
Devices designed for overhead immersion into substances; ideal for pulverization of plant/animal tissue, grain/seed, sample prep, and more. Includes accessories such as generator probes of varying tips, stand assemblies, bead mill tubes, and more.
Homogenizers can be used with tissue, plant, food, soil, and other sample types. Homogenization is a common sample preparation step for analyzing nucleic acids, proteins, cells, metabolism, pathogens, and other targets.
Cell fractionation releases the contents of cells, and newer methods have been designed to consider quality and environmental aspects, cross-contamination, aerosol generations, infection risk, and noise levels. Different methods have been developed for disrupting cell walls and other structures. These include bead mills, sonication, rotor-stator mechanical, high pressure, and other physical methods.
For biological work, sonication may adequately disrupt or deactivate biological material via the process of sonoporation. Sonic dismembrators can break up cells and fragment DNA molecules by subjecting the sample to ultrasonic waves that create small fragments.
Some substances can be mechanically homogenized using beads and other small pieces of metal or minerals. The sample is added to a vial that contains these small particles; the tubes are then rapidly agitated, which breaks down the materials. Depending on the equipment, many samples can be processed simultaneously with this method.
Another mechanical homogenization method is the rotor-stator; these devices have a fast-spinning inner rotor and a stationary outer sheath (stator). Samples are subjected to tearing, shear fluid forces, and cavitation (bubble formation and collapse). A rotor-stator processes a single sample at a time; high-throughput and continuous use models are available.
An immersion blender has an electric motor that powers rotating blades at the end of a shaft. The device is lowered into the sample or mixture and the motor activated. The device may be hand-held or clamped to a stand.
Homogenizer packages are also available for motorized methods.
BioSpec Products Mini-Beadbeater-96, 115 volt
The MiniBeadbeater-96 disrupts cells and tissue by violently shaking a sample inside a classic 2 ml microvial or standard deep well microplate partially filled with tiny ceramic or steel beads. Complete cell disruption is achieved in 1 to 3 minutes of beadbeating. A common application is nucleic acid extraction. Up to 400 mg of tissue is added to a vial containing glass or ceramic beads and a nucleic acid extraction solution...the later often being purchased as part of a nucleic acid extraction kit. The synergy of simultaneous mechanical cell disruption and nucleic acid extraction in a chaotropic solution gives very high yields of high quality RNA or DNA. Most protocols developed using smaller capacity models of the MBB are transferable with little if any modification.
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